Kochian L V, Xin-Zhi J and Lucas W J 1985 Potassium transport in corn roots. Spanswick R M and Costerton J W F 1967 Plasmodesmata in. Not logged in Kawabe S, Fukumorita T and Chino M 1980 Collection of rice phloem sap from stylets of homopterous insects severed by YAG laser. Bostwick D E, Dannenhoffer J M, Skaggs M I, Lister R M, Larkins B A and Thompson GA 1992 Pumpkin phloem lectin genes are specifically expressed in companion cells. Shop now! Lucas W J and Wolf S 1993 Plasmodesmata: the intercellular organelle of green plants. Protoplasma 178, 81–85. c. The … Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence. New Phytol. Ishiwatari Y, Fujiwara T, McFarland K C, Nemoto K, Hayashi H, Chino M and Lucas W J 1997 Rice phloem thioredoxin h has the capacity to mediate its own cell-to-cell transport through plasmodesmata. Besides. Plant Physiol. Kochian L V and Lucas W J 1988 Potassium transport in roots. Robards A W and Lucas W J 1990 Plasmodesmata. http://experiment.worldcat.org/entity/work/data/330043#Topic/molecular_probe_techniques_congresses; http://id.worldcat.org/fast/832759 Ding B, Haudenshield J S, Hull R J, Wolf S, Beachy R N and Lucas W J 1992 Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants. Application of microinjection techniques to plant nutrition Application of microinjection techniques to plant nutrition Lucas, William 1997-10-01 00:00:00 To gain a full understanding of the complex processes that underlie plant nutrition requires the elucidation of the genetic, molecular, biochemical, biophysical, … 13, 45–48. Science 256, 663–665. Annu. Plant Cell Environ. Waigmann E, Lucas W J, Citovsky V and Zambryski P 1994 Direct functional assay for tobacco mosaic virus cell-to-cell movement protein and identification of a domain involved in increasing plasmodesmal permeability. II. Wolf S, Deom C M, Beachy R N and Lucas W J 1989 Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit. Trends Biochem. Hedrich R and Schroeder J I 1989 The physiology of ion channels and electrogenic pumps in higher plants. 41, 369–419. Future studies aimed at identifying additional genes associated with this plasmodesmal macromolecular trafficking system will advance our understanding of the function and evolution of this novel plant communication system. Sommer H, Beltran JP, Huijser P, Pape H, Lonnig W E, Saedler H and Schwartz-Sommer Z 1990. Plant Physiol. Figure 8. Sakuth T, Schobert C, Pecsvaradi A, Eichholz A, Komor E and Orlich G 1993 Specific proteins in the sieve-tube exudates of, Schachtman D P, Schroeder J I, Lucas W J, Anderson J A and Gaber RF 1992 Expression of an inward-rectifying potassium channel by the, Schobert C, Großmann P, Gottschalk M, Komor E, Pecsvaradi A and Nieden U Z 1995 Sieve-tube exudate from. Typically, microinjection is performed under a microscope. One end of a glass micropipette is heated until the glass becomes somewhat liquified. Plant Molec. Fisher D B, Wu Y and Ku M S B 1992 Turnover of soluble proteins in the wheat sieve tube. Reynolds, J.M. Expression of exogenous mRNA in Xenopus laevis embryos for the study of cell cycle regulation / Jill C. Sible and Brian N. Wroble --Use of luciferase chimaera to monitor PLC[zeta] expression in mouse eggs / Karl Swann [and others] --Analysis of 14-3-3 family member function in Xenopus embryos by microinjection of antisense … 47, 1119–1128. Cell 76, 925–932. Science 237, 384–389. 34, 927–933. "Chapters cover all of the hot topics in which the technique can be used (namely, all the research field you are involved!) High–quality microinjection results require precision and reliability. 15, 93–178. ...you'll find more products in the shopping cart. This is a preview of subscription content, Anderson J A, Huprikar S S, Kohian L V, Lucas W J and Gaber R F 1992 Functional expression of a probably. Microinjection processes use either metal microinjection needles or glass micropipettes to inject small liquid volumes. Phytopathol. Borstlap A C 1981 Invalidity of the multiphasic concept of ion absorption in plants. Inverted microscopes are generally used for microinjection applications. Springer-Verlag, Berlin, Germany. © 2020 Springer Nature Switzerland AG. For example, genetic material may be inserted into a living cell, a drug introduced into an eye or brain, or fluid injected into a muscle. Plant Mol. Nakamura S, Hayashi H, Mori S and Chino M 1993 Protein phosphorylation in the sieve tubes of rice plants. Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research. 36, 17–27. Noueiry A O, Lucas W J and Gilbertson R L 1994 Two Proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. Plant Cell 4, 1539–1548. Plant Mol. 114, 305–340. Microinjection. Virology 207, 345–353. Münch E 1930 Die Stoffbewegung in der Pflanze. Annu. The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. 21, 1319–1327. Wolf S and Lucas W J 1997 Plasmodesmal-mediated plant communication network: Implications for controlling carbon metabolism and resource allocation. 40, 539–569. Libros Los más vendidos Infantil y Juvenil Literatura y Ficción Libros de texto Negocios e Inversiones Tienda de Comics Tienda Kindle Los más vendidos Infantil y … This Application Note details our microinjection workflow for CRISPR/Cas9 mediated knock-out and knock-in approaches using the Eppendorf microinjection system. Table 7: Applications of microinjection. Clark, J. Mottley, P.L. http://schema.org/about. (gross), © 2020 Springer Nature Switzerland AG. Lucas W J, Ding B and van der Schoot C 1993a Plasmodesmata and the supracellular nature of plants. The microinjection polymer molding market growth is driven by demand for micro gears, replacing affordable feedstock like PEEK. 114, 475–481. ... April 1992, cell biologists explore how the microinjection of defined material can be used to manipulate the fundamental biochemistry of specific living cells. As example for a knock-out approach, we address the loss of the oculocutaneous albinism 2 (oca2, Lischik et al., 2019) Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Peter H. Bach, C.H. Plant Physiol. Proc. 70, 1723–1731. Science 270, 1980–1983. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Bach, P H, European Workshop on … The application of microinjection methods, in conjunction with molecular tools, established a powerful experimental approach to elucidate the processes underlying plant growth and development. Plant J. enable JavaScript in your browser. Natl. Bot. We have a dedicated site for United Kingdom. Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. The application of microinjection methods, in conjunction with molecular tools, established. Plant Cell Environ. This video has introduced the microinjection setup, demonstrated how to prepare microinjection needles, shown how to prepare embryos for microinjection, perform the microinjection technique, and some applications of microinjection. Plant Molec. Happy Holidays—Our $/£/€30 Gift Card just for you, and books ship free! Cite as. price for Spain Bot. Trends in Cell Biol. Rev. Rev. USA 91, 1433–1437. USA 92, 3814–3818. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Rev. Sci. Sci. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Proceedings of the First European Workshop Held in … In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. pp 25-39 | This service is more advanced with JavaScript available, Plant Nutrition for Sustainable Food Production and Environment Resolution of kinetics into a saturable and a linear component. Annu. Lucas W J, Bouche-Pillon S, Jackson D P, Nguyen L, Baker L, Ding B, and Hake S 1995 Selective trafficking of KNOTTED1 and its mRNA through plant plasmodesmata. What’s New In Plant Physiol. Microinjection Microinjection is a technique of delivering foreign DNA into a living cell (a cell, egg, oocyte, embryos of animals) through a glass micropipette. Biol. Planta 190, 88–96. Planta 195, 456–463. 73, 208–215. 32, 251–273. Polymer Microinjection Molding Market Types, Regions and Applications Research Report Forecast to 2027 : Accu-Mold LLC, ALC Precision, Stamm AG, Sovrin Plastics, Etc. Tester M 1990 Plant ion channels: whole-cell and single-channel studies. 28, 495–502. C. H. Reynolds. Ding B, Qiubo L, Nguyen L, Palukaitis P and Lucas W J 1995 Cucumber mosaic virus 3a protein potentiates cell-to-cell trafficking of CMV RNA in tobacco plants. Hedrich R Schroeder J I and Fernandez J M 1986 Patch-clamp studies on higher plant cells: a perspective. As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. The techniques described tend to be highly … Over 10 million scientific documents at your fingertips. dextran - fluorescein isothyocyanate conjugated dextran. Planta (In Press). Wolf S, Deom C M, Beachy R and Lucas W J 1991 Plasmodesmatal function is probed using transgenic tobacco plants that express a virus movement protein. In cases where very small volumes need to be injected (less than 2 nanoliters), gas-pressure regulated microinjectors may be used. Proc. Rev. Olesinski A A, Lucas W J, Galun E and Wolf S 1995 Pleiotropic effects of TMV-MP on carbon metabolism and export in transgenic tobacco plants. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence: Amazon.sg: Books Ishiwatari, Y, Honda C, Kawashima I, Nakamura S, Hirano H, Mori S, Fujiwara T, Hayashi H and Chino M 1995 Thioredoxin h is one of the major proteins in rice phloem sap. DNA microinjection methods are used to transfer genes between animals and are a popular technique for creating transgenic organisms, particularly mammals. 125, 435–476. 4, 189–195. (CarloAlberto Redi, European Journal of Histochemistry, October, 2009), Expression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation, Use of Luciferase Chimaera to Monitor PLCζ Expression in Mouse Eggs, Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection of Antisense Morpholino Oligos, A Microinjectable Biological System, the Xenopus Oocyte, as an Approach to Understanding Signal Transduction Protein Function, Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs, Analysis of Signaling Pathways in Zebrafish Development by Microinjection, Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complementary Approaches to Study Protein Function, DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines, Bacteriophage φC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels, Germline Transformation of Caenorhabditis elegans by Injection, Quantitative Microinjection of Mouse Oocytes and Eggs, Microinjection of Follicle-Enclosed Mouse Oocytes, Functional Studies of Regulatory Genes in the Sea Urchin Embryo, Exploring the Cytoskeleton During Intracytoplasmic Sperm Injection in Humans, Somatic Cell Nuclear Transfer in the Mouse. Detailed protocols for a wide range of applications … 111, 541–550. Microinjection is a direct method to introduce DNA into either cytoplasm or nucleus. Lucas W J, Balachandran S, Park J and Wolf S 1996 Piasmodesmal companion cell-mesophyll communication in the control over carbon metabolism and phloem transport: insights gained from viral movement proteins. A borosilicate glass capillary is heated and drawn out, then bent using forceps to produce a needle tip of around 10-30 micrometers. Bush D R 1993 Proton-coupled sugar and amino acid transporters in plants. Microinjection is frequently used in genetic engineering andtransgenetics to insert genetic material into single cells. Characterization of the linear component. Kochian L V and Lucas W J 1982b A re-evaluation of the carrier-kinetic approach to ion transport in roots of higher plants. Lucas W J, Olesinski A, Hull R J, Haudenshield J, Deom C M, Beachy R N and Wolf S 1993b Influence of the tobacco mosaic virus 30-kDa movement protein on carbon metabolism and photosynthate partitioning in transgenic tobacco plants. Microinjectors For All Your Microinjection Applications Designed to simplify intracellular injection and a variety of other microinjection tasks, the WPI introduces three new Microinjectors: Type MICRO-ePUMP uPUMP PV850 Regulated compensation and injection pressure Yes Yes Yes Pressure output: 0.3–87 PSI Yes … 2. Biol. Goodwin P B 1983 Molecular size limit for movement in the symplast of the, Goodwin P B and Lyndon R F 1983 Synchronisation of cell division during transition to flowering in. Science 246, 377–379. a A flow of air is blown from the left, and the tip of a 1 ~ micropipette is closed by approaching it with the wann filament b. I. Plant Mol. (Ed.). Olesinski A A, Almon E, Navot N, Galun E, Lucas W J and Wolf S 1996 Tissue specific expression of tobacco mosaic virus movement protein in transgenic potato plants alters plasmodesmal function and carbohydrate partitioning. Plant Nutrition for Sustainable Food Production and Environment, Section of Plant Biology, Division of Biological Sciences, https://doi.org/10.1007/978-94-009-0047-9_3. Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence Edited by P. H. Bach published on October, 2012: Amazon.es: Libros Kochian L V and Lucas W J 1983 Potassium transport in corn roots. Other applications of micro molded parts in automobile and drive systems consists ofmicro gear … Acad. Plant Physiol. Lucas W J 1995 Plasmodesmata: intercellular channels for macromolecular transport in plants. Plant Physiol. Process is applicable for plant cell as well as animal cell but more . It is quickly stretched which forms a very fine tip at the heated end. Plant Physiol. Nakamura S, Hayashi H, Mori S and Chino M 1996 Detection and characterization of protein kinases in rice phloem sap. To gain a full understanding of the complex processes that underlie plant nutrition requires the elucidation of the genetic, molecular, biochemical, biophysical, physiological and environmental factors that interact, at the cellular, organ and whole plant levels, to allow this sessile organism to optimize the allocation and utilization of available resources. Lucas W J and Gilbertson R L 1994 Plasmodesmata in relation to viral movement within leaf tissues. Plant Cell Physiol. Plant Cell Physiol. Jackson D, Veit B and Hake S 1994 Expression of maize. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. One of the most widely used applications of microinjec… Deom C M, Oliver M J, and Beachy R N 1987 The 30-kilodalton gene product of tobacco mosaic virus potentiates virus movement. Gustav Fischer, Jena. Erwee M G and Goodwin P B 1983 Characterisation of the, Erwee M G and Goodwin P B 1984 Characterization of the, Erwee M G and Goodwin P B 1985 Symplast domains in extrastellar tissues of, Erwee M G, Goodwin P B and Van Bel A J E 1985 Cell-cell communication in the leaves of. Karrer E E, Lincoln J E, Hogenhout S, Bennett A B, Bostock R M, Martineau B, Lucas W J, Gilchrist D G and Alexander D 1995 In situ isolation of mRNA from individual cells: creation of cell-specific cDNA libraries. This has the potential to expand the application of microinjection to other procedures, such as production of over-expressed proteins for western blot, immunoprecipitation, purification, and bioluminescence, where they are critically needed but cannot be implemented due to inherent drawbacks of … Biol. a powerful experimental approach to elucidate the processes underlying plant growth and development. Unable to display preview. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Balachandran S, Xiang Y, Schobert C, Thompson G A and Lucas W J 1997b Phloem sap proteins of, Behrens H M, Gradmann D and Sievers A 1985 Membranepotential responses following gravistimulation in roots of. 32, 387–411. Sussman M R 1994 Molecular analysis of proteins in the plasma membrane. The First European Workshop on Biotechnology Applications of Microinjection, Microscopic Imaging and Fluorescence was run at the University of East London, U.K, 21st-24th April, 1992 with the objective of bringing together a diverse group of individuals who were using these state-of-the-art applications for biotechnological … Plant Physiol. Annu. Natl. Spanswick R M 1972 Electrical coupling between cells of higher plants: a direct demonstration of intercellular communication. Sentenac H, Bonneaud N, Minet M, Lacroute F, Salmon J-M, Gaymard F and Grignon C. 1992 Cloning and expression in yeast of a plant potassium transport system. J. Exp. It is a microsurgical procedure conducted on a single cell, using a glass needle (i.e., a fine, glass microcapillary pipette), a precision positioning device (a micromanipulator) to control the movement of the micropipette, and a microinjector. In rice phloem sap W 1976 intercellular communication 4r or InjectMan ® 4 they form ideal... 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